The new product Illumina Ribo-Zero Plus rRNA Microbiome Depletion Kit is a robust, effective, and simple method to reduce rRNA content for high-quality microbial transcriptome analysis. The kit meets or exceeds Ribo-Zero Plus performance metrics in all the tested microbial isolates and “microbiome” mock community samples. The Illumina Ribo-Zero Plus Microbiome kit is compatible with low inputs, facilitating meta-transcriptomic analysis of low biomass samples.

This new methodology facilitates rich transcriptome analyses of microbial isolates. The single-tube enzymatic ribodepletion method reduces rRNA in both prokaryotic and eukaryotic samples. Abundant rRNA is removed from total RNA by targeted hybridization to DNA probes and subsequent RNase H-mediated cleavage. Ribo depleted samples then undergo library preparation, sequencing, and data analysis with a no-cost analysis software on BaseSpace.

Analysis Software: Microbiome Metatranscriptome.

Sample: Stool, Skin and Oral.

Use in the equipment: NextSeq 1000/2000 and NovaSeq.

Sample input: 50-100 ng.